A strain of Peudomonas putida capable of producing phytotoxic metabolite was investigated for its biocontrol activity against Chromolaena odorata, Tridax procumbens and Physalis angulata. The organism was mass produced separately in fermentation media and phytotoxic metabolite was precipitated from the culture. Bioherbicide formulation was produced from metabolite viz the bioherbicide formula (which is made up of ten percent of the metabolite yield dissolved in water to make a total of 100 ml). The formulation was sprayed on the three weeds and their effects on the leaves of the three weeds were expressed as percentage and shown pictorially. The volume of the phytotoxic materials obtained from organism was 20ml. The bioherbicide formular obtained from- P. putida showed complete withering of the Chromolaena weed by the 8th day while it took 9 days for Physalis and Tridax to be completely withered after application. Hence, it has therefore been shown that Phytotaxic metabolic of P. putida is efficacious and safe and therefore can be used as an alternative to chemical herbicide and can therefore be optimized for large scale field application.